Insertion junction: CLIP2.014051_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.014051
Chromosome:chromosome 2
Location:2722376
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCCCTGTTGACAGCTGCCCTCGAGAGCTTCGTGCCGGAGTCTGTTGGC
Internal bar code:GAGTTTTATGCCAAGGTAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1001
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAAGTCCAGGGCAGACAT
Suggested primer 2:GTCATTCCCGAACCAGACGT