Insertion junction: CLIP2.014054_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.014054
Chromosome:chromosome 1
Location:3047961
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g019300 Nucleotidyl cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCGGCAACCGGGCGCGCACCCAACGGCATTGCGGCAGGCGGGGCTAT
Internal bar code:TTGATCAAATTGTTCGTAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2710
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGGATGCTTTGCTTGTGC
Suggested primer 2:CCTCGACGCCGTTATCATCA