Insertion junction: CLIP2.014057_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014057
Chromosome:chromosome 2
Location:3615656
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g099750 (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGAAGAGAGAGGGCGGGTCGTTGTCTAACCGCTCACAAATATACTCGA
Internal bar code:TTGCATTCCGTTACCAAATGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1870
LEAP-Seq percent confirming:92.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCATGAGAACCGAGGATG
Suggested primer 2:CAGGAAAGCAAACCACACCG