Insertion junction: CLIP2.014057_4

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.014057
Chromosome:chromosome 11
Location:32878
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCCCACTGAGCCGTCACCCAGCCACCCAGCCATCACCCAGCCAGCCGC
Internal bar code:AGTGATCTAGTTGGGGATATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1123
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGATGCATCCACACCAA
Suggested primer 2:CAATCGCGATGTCACAGCAG