| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.014064 |
| Chromosome: | chromosome 17 |
| Location: | 2361665 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713900 | LST8 | TOR kinase binding protein; (1 of 1) K08266 - G protein beta subunit-like (GBL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAATAGATGGAGGTGCGGCTCCTGTGGCCAAACTGACGTCTAACGCTA |
| Internal bar code: | GCTTAATTCGCCGTTTTCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3535 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGCTACTGACGTGCGTT |
| Suggested primer 2: | GGCAGTCGGGTGATATGAGG |