Insertion junction: CLIP2.014074_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.014074
Chromosome:chromosome 10
Location:2440099
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g435350 ESU1,ESU1a Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATGCGCACCGGTTTCCCTGTCAAGTTTTCTTAGTACACGGACCCGCCG
Internal bar code:GCAGGAGGATAAAGAGCGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1944
LEAP-Seq percent confirming:97.3684
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCGTAGGCCTACTTCTC
Suggested primer 2:ATGCACACGGAGGTCAGAAG