| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.014087 |
| Chromosome: | chromosome 12 |
| Location: | 9526801 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540200 | HEL58 | (1 of 5) K12823 - ATP-dependent RNA helicase DDX5/DBP2 (DDX5, DBP2); DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCCGCAGCAGCTGCACCCCCCGCCGCCTTGTCTGCCGCCGCGGCCTT |
| Internal bar code: | TACCGTAAGATATGTTATTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1103 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGATGGCCAAGCAGCAAC |
| Suggested primer 2: | ATAGTTGCAGTGCACCGTCA |