Insertion junction: CLIP2.014111_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014111
Chromosome:chromosome 9
Location:3556898
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g396624 (1 of 1) IPR000104//IPR001841//IPR002110//IPR016024//IPR020683 - Antifreeze protein, type I // Zinc finger, RING-type // Ankyrin repeat // Armadillo-type fold // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGAAGGCCTCCGCGACGTGGCCCCACTGTCCCGCGCACATCCGTCCAC
Internal bar code:GTGTGTGCTCGTCCCATGCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1700
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCATCCTTGGATCTAGC
Suggested primer 2:ACCTGGAATCCGAGATCCCA