Insertion junction: CLIP2.014116_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.014116
Chromosome:chromosome 4
Location:3736954
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g229350 (1 of 1) 2.1.1.290 - tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase / tRNA-yW synthesizing enzyme-4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCAGCCGCGCTCCGCCAGGTTGCACACCATCTGGCGCCCGAACGCATC
Internal bar code:CTGGCGGCGGATAGGGGGCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3668
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCTTGCCTAGAACTGCCG
Suggested primer 2:TACAGGCAAGTTGACCGTCC