| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.014150 |
| Chromosome: | chromosome 17 |
| Location: | 6548378 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744947 | (1 of 1) 2.1.1.230 - 23S rRNA (adenosine(1067)-2'-O)-methyltransferase / Thiostrepton-resistance methylase | CDS | |
| lncRNA_TCONS_00240319 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACACTTACACCGGGTCCTGGTGGGGCCAGCCTTCAAGCGGCTCTGGG |
| Internal bar code: | GGTTGGGAATAGTCGAGGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATCGTCCATGTCGTTGT |
| Suggested primer 2: | ACGTTCCGGCATGTATGGTT |