| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.014176 |
| Chromosome: | chromosome 13 |
| Location: | 2496233 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579850 | (1 of 1) PTHR10984//PTHR10984:SF25 - ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN // ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3 | 3'UTR | |
| Cre13.g579901 | DEG6 | (1 of 2) PTHR22939:SF67 - PROTEASE DO-LIKE 9; Deg protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCGCGGCGTCGACACTCCAGCGGACCAGCCCGATTGCGACCGTGA |
| Internal bar code: | AGAACTTCCGGGATCGCCTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1572 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAGCTGGGCAAGTTCTC |
| Suggested primer 2: | TGTGACCCTCAACATGACCG |