| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014210 |
| Chromosome: | chromosome 6 |
| Location: | 1932351 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264450 | AOT5 | Amino acid transporter; (1 of 1) K14994 - solute carrier family 38 (sodium-coupled neutral amino acid transporter), member 7/8 (SLC38A7_8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCGAGCCACCTGCGGGTGTGCGGGGGCGGTGTTGGCAGGTGGGAGG |
| Internal bar code: | CTCCCGGCAGCGTCGACTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGGGGAATGCGTCTAAA |
| Suggested primer 2: | GCCCTTCTTGATGCCCTTCT |