Insertion junction: CLIP2.014247_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.014247
Chromosome:chromosome 1
Location:5243260
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g036750 DMA1 (1 of 1) 2.1.1.72 - Site-specific DNA-methyltransferase (adenine-specific) / Restriction-modification system; Putative DNA methylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACCCCAAGCGGAGCAACATCAACCAGATTGCACATCTCTACGCTGCGC
Internal bar code:CTCGTAAGACACCCCCAAGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4674
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:81

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCGCTTTTACTTGATCCG
Suggested primer 2:GCAAGAAGCGTGGCTGAAAA