| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014250 |
| Chromosome: | chromosome 3 |
| Location: | 3861838 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170400 | (1 of 1) PF12579 - Protein of unknown function (DUF3755) (DUF3755) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAATCCTTTTTGCCAATTTTGCGTCGTGTACCCACAGTTCACGCCAGT |
| Internal bar code: | CCCAGCGGCGGAGGTAGTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3355 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCGATAGCGCATGAGAA |
| Suggested primer 2: | ATACTCCTAGTGGCCCAGCA |