Insertion junction: CLIP2.014257_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.014257
Chromosome:chromosome 10
Location:2517345
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g436000 POC2 Proteome of centriole protein 2; (1 of 2) PTHR10877//PTHR10877:SF135 - POLYCYSTIN-RELATED // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGGAGGATCGGCTGTACTACACGGCCAATGAGAAGAACGCCACGCGCG
Internal bar code:TTTTGCGAGGAGGGGGGAAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3685
LEAP-Seq percent confirming:98.7952
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:CCAATCTCTGCCATTTCGCG
Suggested primer 2:CAGAGCATCCAGAGCGTAGG