| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014272 |
| Chromosome: | chromosome 9 |
| Location: | 35887 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386119 | (1 of 2) PF13383 - Methyltransferase domain (Methyltransf_22) | CDS | |
| Cre09.g386131 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGCATTGGAGGCTCGGCTTCGGTACTCGACATGGATCGGACGGCGTC |
| Internal bar code: | GTGCTCCCGGACAACGGATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3661 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 141 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 141 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATGTGTACAGCTGCGGT |
| Suggested primer 2: | CGTGCCTTCAAATATGCCCG |