| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.014304 |
| Chromosome: | chromosome 16 |
| Location: | 6632032 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673700 | (1 of 1) K14799 - pre-rRNA-processing protein TSR1 (TSR1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCACCACTGCCGTCCGCCGCCGCCGCCCCCTCAGGCAGCTTGAAGG |
| Internal bar code: | CTAGGGGGATAGATGAAATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2234 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCATGGAGAAGTTCCTG |
| Suggested primer 2: | GGACAAGTGCAGCAGAGGAT |