Insertion junction: CLIP2.014305_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.014305
Chromosome:chromosome 8
Location:2773757
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g373583 OXA2,ALB3D (1 of 1) IPR000104//IPR001708//IPR013026//IPR019734 - Antifreeze protein, type I // Membrane insertase OXA1/ALB3/YidC // Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat; ALBINO3-like translocon protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCTCCTACCTCTACCCCTAAGCCCCTACCCCTACGGCCCTACCCCTA
Internal bar code:CCTAGGAGGAACAACAACCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:961
LEAP-Seq percent confirming:77.7778
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGTACACCTCGCTCGTAC
Suggested primer 2:AGATGGGGGTTGCGTTGTAG