Insertion junction: CLIP2.014320_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.014320
Chromosome:chromosome 16
Location:5996244
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g678551 CYG34 (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGACCACCACCTCGTGCCATACCTCTGGCATCTCCAGGGCCCCATCC
Internal bar code:TATGACAGAGCTAGAGCATCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:5593
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGGCGCAAGTCTCAGAG
Suggested primer 2:CGTTTGTTTGAGAGCGCACA