Insertion junction: CLIP2.014332_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.014332
Chromosome:chromosome 9
Location:2015729
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g396750 CSE18 Predicted protein of CSE family; (1 of 1) IPR000601//IPR022409 - PKD domain // PKD/Chitinase domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTTTCTCGGATCCAGGATCCGCTGGCTCGCCATCCGGCCAGATCTGG
Internal bar code:ACAATCCAAGGGTGCCGGACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3007
LEAP-Seq percent confirming:92.5926
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGCAGAAGACAGTCACC
Suggested primer 2:GGTGGTAAGGAAGGCAGGTC