| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.014336 |
| Chromosome: | chromosome 16 |
| Location: | 3748196 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683793 | (1 of 1) K14843 - pescadillo (PES1, NOP7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGGGAAAAGTCTGGCGGCGTGTTGGGCTGAGCCAGGCGCGACCCGAG |
| Internal bar code: | CAGATGCAAACCACTGATGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAAGCGCGGTTGCCATAG |
| Suggested primer 2: | ATGGTGTGCTGGGGTAGTTG |