| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.014350 |
| Chromosome: | chromosome 16 |
| Location: | 1137359 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649900 | HTR40,HTR24 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACACAAGAGCCGCAGCAAACGCCTGACTTATCCTGCGCCAAGCAGCA |
| Internal bar code: | AGGCTTCCGCTTTGCGATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2826 |
| LEAP-Seq percent confirming: | 94.8276 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCTACCCCCTAACCC |
| Suggested primer 2: | TCCAGGAACGTCTTCAGCAC |