Insertion junction: CLIP2.014370_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.014370
Chromosome:chromosome 6
Location:4246439
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278251 ACRY1,aCRY (1 of 1) K02295 - cryptochrome (CRY); Animal-like Cryptochrome CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACACGGTGCGGCGCGACGCGGCGCGAAGCGACGATGTGGTTTGGCATG
Internal bar code:TCAATGGAGTGTGTTGATAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:411
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAACTCGGGGTTGTCGTC
Suggested primer 2:CTCAAACCAGGCTAACCGGT