Insertion junction: CLIP2.014375_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.014375
Chromosome:chromosome 1
Location:4056543
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800078 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGGTGGAGATGCTCATCATCAACTGGCTGCACGCGGCGCGGCTGGGCG
Internal bar code:ACGAGGCGTAGGATTGGTACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1073
LEAP-Seq percent confirming:26.9231
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACCCATGTAGACACAG
Suggested primer 2:TGCCCCGAGAAGATTGCATT