| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014390 |
| Chromosome: | chromosome 1 |
| Location: | 4677132 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032400 | (1 of 1) PF05118 - Aspartyl/Asparaginyl beta-hydroxylase (Asp_Arg_Hydrox) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTTGGGTGGGTTTTGTAAAATGAGCACAAGGAAAACAATAAGCTCA |
| Internal bar code: | CAACCAGTACGTAAGCTCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAAGCCTCAGACCCGTAC |
| Suggested primer 2: | TCTTCCCTGGCGACTTTGAC |