| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.014407 |
| Chromosome: | chromosome 3 |
| Location: | 3425036 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166800 | (1 of 1) PTHR10877//PTHR10877:SF130 - POLYCYSTIN-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACGGGCGGCAGCGGCGTCGGCGGCGGCATCGGTGGTGTCGGGCCGA |
| Internal bar code: | GGGTATTTCTAAGTGCGTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2787 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCCAGGTTACTCGCAAG |
| Suggested primer 2: | ATGGACAGTACGTTGGCTGG |