Insertion junction: CLIP2.014409_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.014409
Chromosome:chromosome 16
Location:4327629
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g668950 CPZ (1 of 1) 3.4.17.1 - Carboxypeptidase A / Carboxypolypeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCGGCGCCGATGCCGAGGAGGCGGAGGTGGAGGCGGAGGTGGAGGCGG
Internal bar code:AATCGAACATATTCGTGTTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2303
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTACAGCAGGATGACCACCC
Suggested primer 2:GGACCACGTCAACTATGGCA