Insertion junction: CLIP2.014410_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.014410
Chromosome:chromosome 9
Location:4638690
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401812 (1 of 1) PF00580//PF13086//PF13087 - UvrD/REP helicase N-terminal domain (UvrD-helicase) // AAA domain (AAA_11) // AAA domain (AAA_12) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTAGGCCTCCCGCTCCTCCGCCCCACTGCTCACGTCCAGCACCACCAG
Internal bar code:AACATTGGGAACAACTTGAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2701
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAGACGATGCAAATCCGC
Suggested primer 2:CCCCTGTTGCTCGTCTTCTT