Insertion junction: CLIP2.014411_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.014411
Chromosome:chromosome 12
Location:9436233
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g541100 OTU7 (1 of 1) PTHR12419//PTHR12419:SF19 - OTU DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED; OTU-like cysteine protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGCTGGTCCCGCCCGGCGCGCGGCACCAACGCAGCTACTCTGACGGGA
Internal bar code:GCTCATGTATTTATTCCCAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:615
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCATGCACATACACGCAC
Suggested primer 2:AAGGCGGTGATCCAAGTGAG