Insertion junction: CLIP2.014413_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014413
Chromosome:chromosome 10
Location:3035466
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g440850 GPX4 Glutathione peroxidase 4; (1 of 2) 1.11.1.12 - Phospholipid-hydroperoxide glutathione peroxidase / Peroxidation-inhibiting protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTATAGATCGCCCCCTCAACATCCCCCTGGTCCCAGTTGTGGTAAAAC
Internal bar code:CTTTAAACCACTCGAAAGTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2814
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTGCACTCAGATCTTGT
Suggested primer 2:TGACACTCTGTGCGTGGTAC