Insertion junction: CLIP2.014418_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.014418
Chromosome:chromosome 8
Location:1705106
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g366200 (1 of 1) K17983 - sensitive to high expression protein 9, mitochondrial (SHE9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCGCGTCTCCACGCCCTCTAGCCGCTGCCTCAGCCCGCGCATCTCCT
Internal bar code:CGCTAGTACTTTACCATCGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:126
LEAP-Seq percent confirming:2.63158
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGGCCTCATTCATGGTG
Suggested primer 2:AACGCACAGAACGCACTAGA