Insertion junction: CLIP2.014419_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014419
Chromosome:chromosome 6
Location:4975055
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g281400 NAH1 Cation antiporter; (1 of 11) PF00999 - Sodium/hydrogen exchanger family (Na_H_Exchanger) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCACGTTGTTGGCGTCGTCAGTGGCCAGCAGGGCACGGCGGTCAGTCA
Internal bar code:AGGATAGGTTACTCTTGGACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1782
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AGATGATCCACGTGAGCAGC
Suggested primer 2:CGCTTGCACACACATGTTGA