| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.014439 |
| Chromosome: | chromosome 2 |
| Location: | 5738187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116650 | (1 of 7) 3.5.1.52 - Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase / N-oligosaccharide glycopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGACGTGGACTCACCGCGCAGCTCGCCGTGAGCGAACAGCACCCCCAA |
| Internal bar code: | TGTGATTTGGTCTAGGCCATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2675 |
| LEAP-Seq percent confirming: | 90.3226 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTTCGCCTGTGTTCTA |
| Suggested primer 2: | GGTCAGTCATGGGGCATTCA |