| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014461 |
| Chromosome: | chromosome 16 |
| Location: | 938699 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648451 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAAGGCAAAGAGGCACGTGTTGGAGCCGTCGGGTGCACGTGCGCGGCA |
| Internal bar code: | GCTAATTTCTGTTTACAAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2917 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGACCGGACTTACACAC |
| Suggested primer 2: | GGTCGTGTTGTTGTTGGTGG |