Insertion junction: CLIP2.014468_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.014468
Chromosome:chromosome 7
Location:2924212
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g332700 (1 of 1) PF00069//PF13639 - Protein kinase domain (Pkinase) // Ring finger domain (zf-RING_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCAGGTGGGCGTGCACAAGCGCGCCCCAGACGTCCCCACGCACGCGCC
Internal bar code:TCGTGGGTATTCAGTCCCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2051
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTAGAGCACCACCGATAC
Suggested primer 2:GTGGTGATGGCTGACCTCAA