Insertion junction: CLIP2.014557_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.014557
Chromosome:chromosome 3
Location:5469839
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g184400 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase; Putative NAD+ diphosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGAAAATTGTGACGTAGTGGGTGGTGGCGTTGAAGACGGTGTTTGTTG
Internal bar code:GCGGTAAAGGTAGATGACGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2693
LEAP-Seq percent confirming:85.3659
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGGCTCTCATGGATCCG
Suggested primer 2:TTGGACACACCTGGACACAC