| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014564 |
| Chromosome: | chromosome 12 |
| Location: | 4928019 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524200 | SPC25 | (1 of 1) K12947 - signal peptidase complex subunit 2 (SPCS2, SPC2); Signal Peptidase, 25 kDa catalytic subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCACGCGCGGGCGAGGGAGCCCCCTGCGTACCGGCCTGCTGCTCCAG |
| Internal bar code: | TGAGCCAGGAGCTAAACTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1889 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGAAATTCCGTCAGCGC |
| Suggested primer 2: | TGCTCAACCTGTACAGCTGG |