Insertion junction: CLIP2.014568_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014568
Chromosome:chromosome 16
Location:1400772
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g652150 FBP4 (1 of 1) K01103 - 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGGAGACGTCGGCGGCCGGCAGCCCCGTGTCACTGCACAGCCGCGCG
Internal bar code:TTCTACTTGATGTTCCAGGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2517
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACCCCAACCCCAACAT
Suggested primer 2:CTGAGCATGGGCATGGGTAT