| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014582 |
| Chromosome: | chromosome 2 |
| Location: | 5958264 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118050 | CYCU1 | U-type cyclin; (1 of 1) PTHR15615:SF20 - CYCLIN FAMILY PROTEIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGTTTGACTAGTTTCTGTGAGGCCGGGCATCCTTTATTGCTTGGGTGC |
| Internal bar code: | GCCGCTGGGGGGCCTAGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4246 |
| LEAP-Seq percent confirming: | 82.6531 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCGCGAACTCGTATCGA |
| Suggested primer 2: | CTGCACTTGGTGAACTTGGC |