Insertion junction: CLIP2.014604_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.014604
Chromosome:chromosome 10
Location:3381775
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g443750 (1 of 8) IPR003117 - cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACAACGAATGTTGACAAAGTCGTCAAGGAGATGGTTGTGTCGGTGCTT
Internal bar code:CAAGAGTCTTTTAGCTCAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2568
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTCGTGTCTCTGGTTTGG
Suggested primer 2:CACTGATCGATGCTTTGCCG