Insertion junction: CLIP2.014617_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014617
Chromosome:chromosome 1
Location:5504834
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g039050 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAAGCAACTGATTGCGGTCGCACCCCGCCGTGCAGCGGCGATTGCGGC
Internal bar code:ACACAAGGTCGACATTACGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3814
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:GACGGCTGTCGCTTTCTTTC
Suggested primer 2:CCGCGATGATGACTACACCA