Insertion junction: CLIP2.014620_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.014620
Chromosome:chromosome 6
Location:2396286
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g268550 GTR,CGL121,CSL1 Cellulose-synthase-like 1; (1 of 1) 2.4.1.32 - Glucomannan 4-beta-mannosyltransferase / Glucomannan-synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCGAAGCTACCGTGTCTGTATACCTGAGCGACTGCGTCCCGTGATCCC
Internal bar code:CAACGACAGCTTCTGCTTATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:168
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAGTGTGTGCCACCTATT
Suggested primer 2:AACCCACCCACACACCATTT