| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.014621 |
| Chromosome: | chromosome 14 |
| Location: | 2397313 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGCACCGCACCCGGCATGAGCTTTGGACTGCCCCACCCGCACAGAC |
| Internal bar code: | GTATTTTCAAGCAAATGTTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 68.1818 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACTGCAGACCAAGTCGT |
| Suggested primer 2: | GAAGCCGTTCATGGGGAGAA |