| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014634 |
| Chromosome: | chromosome 6 |
| Location: | 1813549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263500 | (1 of 1) PF01951 - Archease protein family (MTH1598/TM1083) (Archease) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGTAGTCACCGACCGCCGCCGATCGGTACTCCGGCAGGGGCTCCAAC |
| Internal bar code: | GTTTAAGATTCACTAAGTAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2438 |
| LEAP-Seq percent confirming: | 98.8235 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTACGGAGTGCACTGTCA |
| Suggested primer 2: | CCGCCTTCCCAACCATATGT |