| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014640 |
| Chromosome: | chromosome 6 |
| Location: | 8876536 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311700 | FAP349 | Leucine-Rich Repeat Flagellar Associated Protein 349 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGTGGTTGGGGCACCTCAGTTCCTGGTTGCAAACCCCCCCAAAATCCC |
| Internal bar code: | TATTCAGGTCATGATATCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1383 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCATGATTTGAGACTGG |
| Suggested primer 2: | CATTGGCCACCCATACCCTT |