| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.014642 |
| Chromosome: | chromosome 7 |
| Location: | 159026 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313185 | (1 of 1) K03012 - DNA-directed RNA polymerase II subunit RPB4 (RPB4, POLR2D) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGCTTAGGTCAGCGCTGCGTGAGGGCTGGTACCGTTCACAGCAACG |
| Internal bar code: | AAATAGTGGATTTTGTGGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1526 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAACAACTTGAGGGGCG |
| Suggested primer 2: | CACCTTGAGTTCTCCCCACC |