| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014684 |
| Chromosome: | chromosome 7 |
| Location: | 3750864 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338200 | (1 of 2) K17579 - protein phosphatase 1 regulatory subunit 42 (PPP1R42, LRRC67) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGCTGCGGCGTTAGGACTCCACTGAAGGTGCAATTTCACCAGCTCAC |
| Internal bar code: | CTACGATTTATGTTTTACGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1436 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAAGCAGAACGAGTGTG |
| Suggested primer 2: | AGGGAGGCAGGATGTGAGAT |