| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014688 |
| Chromosome: | chromosome 17 |
| Location: | 327473 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698200 | MMS21 | SUMO ligase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGCTCAGCCTGAAGAACGTGAGCGAGGGAGAGGGAGAGGGCGCAGC |
| Internal bar code: | ATTGGAGAACTTATCGTTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 65.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGATTAATGGGGTGGCA |
| Suggested primer 2: | AGCAGGAGGAGGAAGAGGAG |