Insertion junction: CLIP2.014718_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.014718
Chromosome:chromosome 13
Location:2847564
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g583050 Tic55-like5,TIC55-5,PAO8 Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) 3'UTR
Cre13.g583100 (1 of 1) 2.4.99.12 - Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase / Lipid IV(A) KDO transferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGCTTAGACCAAATATCACGTACGCAGCAAGCCAACTTAAATTGGTCA
Internal bar code:GTCAGATGCGGTCTTCTAATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2401
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCTGGTGGCATCAGGTGG
Suggested primer 2:TATGGAGCCCCTGGAATCCA