| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014736 |
| Chromosome: | chromosome 7 |
| Location: | 270643 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313950 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAGTCGGGGTACACCACCTCCTCCCCGTAGACCAGTGAGGGTAGGTA |
| Internal bar code: | GCCTTTGTTAAACCTCGGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAGCACAAGACAGGCAC |
| Suggested primer 2: | ATGTCACTCCACGTTCCACC |